Glycomics of bone marrow-derived mesenchymal stem cells can be used to evaluate their cellular differentiation stage
Identifieur interne : 001323 ( Main/Exploration ); précédent : 001322; suivant : 001324Glycomics of bone marrow-derived mesenchymal stem cells can be used to evaluate their cellular differentiation stage
Auteurs : Annamari Heiskanen [Finlande] ; Tia Hirvonen [Finlande] ; Hanna Salo [Finlande] ; Ulla Impola [Finlande] ; Anne Olonen [Finlande] ; Anita Laitinen [Finlande] ; Sari Tiitinen [Finlande] ; Suvi Natunen [Finlande] ; Olli Aitio [Finlande] ; Halina Miller-Podraza [Suède] ; Manfred Wuhrer [Pays-Bas] ; André M. Deelder [Pays-Bas] ; Jari Natunen [Finlande] ; Jarmo Laine [Finlande] ; Petri Lehenkari [Finlande] ; Juhani Saarinen [Finlande] ; Tero Satomaa [Finlande] ; Leena Valmu [Finlande]Source :
- Glycoconjugate Journal [ 0282-0080 ] ; 2009-04-01.
English descriptors
Abstract
Abstract: Human mesenchymal stem cells (MSCs) are adult multipotent progenitor cells. They hold an enormous therapeutic potential, but at the moment there is little information on the properties of MSCs, including their surface structures. In the present study, we analyzed the mesenchymal stem cell glycome by using mass spectrometric profiling as well as a panel of glycan binding proteins. Structural verifications were obtained by nuclear magnetic resonance spectroscopy, mass spectrometric fragmentation, and glycosidase digestions. The MSC glycome was compared to the glycome of corresponding osteogenically differentiated cells. More than one hundred glycan signals were detected in mesenchymal stem cells and osteoblasts differentiated from them. The glycan profiles of MSCs and osteoblasts were consistently different in biological replicates, indicating that stem cells and osteoblasts have characteristic glycosylation features. Glycosylation features associated with MSCs rather than differentiated cells included high-mannose type N-glycans, linear poly-N-acetyllactosamine chains and α2-3-sialylation. Mesenchymal stem cells expressed SSEA-4 and sialyl Lewis x epitopes. Characteristic glycosylation features that appeared in differentiated osteoblasts included abundant sulfate ester modifications. The results show that glycosylation analysis can be used to evaluate MSC differentiation state.
Url:
DOI: 10.1007/s10719-008-9217-6
Affiliations:
- Finlande, Pays-Bas, Suède
- Götaland, Hollande-Méridionale, Suède occidentale, Uusimaa
- Göteborg, Helsinki, Leyde
- Université d'Helsinki, Université de Göteborg
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Deelder</name><affiliation wicri:level="3"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, Leiden</wicri:regionArea><placeName><settlement type="city">Leyde</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Natunen, Jari" sort="Natunen, Jari" uniqKey="Natunen J" first="Jari" last="Natunen">Jari Natunen</name><affiliation wicri:level="1"><country xml:lang="fr">Finlande</country><wicri:regionArea>Glykos Finland Ltd., Helsinki</wicri:regionArea><wicri:noRegion>Helsinki</wicri:noRegion></affiliation></author><author><name sortKey="Laine, Jarmo" sort="Laine, Jarmo" uniqKey="Laine J" first="Jarmo" last="Laine">Jarmo Laine</name><affiliation wicri:level="1"><country xml:lang="fr">Finlande</country><wicri:regionArea>Research and Development, Finnish Red Cross Blood Service, Kivihaantie 7, 00310, Helsinki</wicri:regionArea><wicri:noRegion>Helsinki</wicri:noRegion></affiliation></author><author><name sortKey="Lehenkari, Petri" sort="Lehenkari, Petri" uniqKey="Lehenkari P" first="Petri" last="Lehenkari">Petri Lehenkari</name><affiliation wicri:level="1"><country xml:lang="fr">Finlande</country><wicri:regionArea>Clinical Research Center, University of Oulu, Oulu</wicri:regionArea><wicri:noRegion>Oulu</wicri:noRegion></affiliation></author><author><name sortKey="Saarinen, Juhani" sort="Saarinen, Juhani" uniqKey="Saarinen J" first="Juhani" last="Saarinen">Juhani Saarinen</name><affiliation wicri:level="1"><country xml:lang="fr">Finlande</country><wicri:regionArea>Glykos Finland Ltd., Helsinki</wicri:regionArea><wicri:noRegion>Helsinki</wicri:noRegion></affiliation></author><author><name sortKey="Satomaa, Tero" sort="Satomaa, Tero" uniqKey="Satomaa T" first="Tero" last="Satomaa">Tero Satomaa</name><affiliation wicri:level="1"><country xml:lang="fr">Finlande</country><wicri:regionArea>Glykos Finland Ltd., Helsinki</wicri:regionArea><wicri:noRegion>Helsinki</wicri:noRegion></affiliation></author><author><name sortKey="Valmu, Leena" sort="Valmu, Leena" uniqKey="Valmu L" first="Leena" last="Valmu">Leena Valmu</name><affiliation wicri:level="1"><country xml:lang="fr">Finlande</country><wicri:regionArea>Research and Development, Finnish Red Cross Blood Service, Kivihaantie 7, 00310, Helsinki</wicri:regionArea><wicri:noRegion>Helsinki</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Finlande</country></affiliation></author></analytic><monogr></monogr><series><title level="j">Glycoconjugate Journal</title><title level="j" type="sub">Official Journal of the International Glycoconjugate Organization</title><title level="j" type="abbrev">Glycoconj J</title><idno type="ISSN">0282-0080</idno><idno type="eISSN">1573-4986</idno><imprint><publisher>Springer US; http://www.springer-ny.com</publisher><pubPlace>Boston</pubPlace><date type="published" when="2009-04-01">2009-04-01</date><biblScope unit="volume">26</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="367">367</biblScope><biblScope unit="page" to="384">384</biblScope></imprint><idno type="ISSN">0282-0080</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0282-0080</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Differentiation</term><term>Exoglycosidase</term><term>Glycomics</term><term>Mass spectrometry</term><term>Mesenchymal stem cells</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Human mesenchymal stem cells (MSCs) are adult multipotent progenitor cells. They hold an enormous therapeutic potential, but at the moment there is little information on the properties of MSCs, including their surface structures. In the present study, we analyzed the mesenchymal stem cell glycome by using mass spectrometric profiling as well as a panel of glycan binding proteins. Structural verifications were obtained by nuclear magnetic resonance spectroscopy, mass spectrometric fragmentation, and glycosidase digestions. The MSC glycome was compared to the glycome of corresponding osteogenically differentiated cells. More than one hundred glycan signals were detected in mesenchymal stem cells and osteoblasts differentiated from them. The glycan profiles of MSCs and osteoblasts were consistently different in biological replicates, indicating that stem cells and osteoblasts have characteristic glycosylation features. Glycosylation features associated with MSCs rather than differentiated cells included high-mannose type N-glycans, linear poly-N-acetyllactosamine chains and α2-3-sialylation. Mesenchymal stem cells expressed SSEA-4 and sialyl Lewis x epitopes. Characteristic glycosylation features that appeared in differentiated osteoblasts included abundant sulfate ester modifications. The results show that glycosylation analysis can be used to evaluate MSC differentiation state.</div></front></TEI><affiliations><list><country><li>Finlande</li><li>Pays-Bas</li><li>Suède</li></country><region><li>Götaland</li><li>Hollande-Méridionale</li><li>Suède occidentale</li><li>Uusimaa</li></region><settlement><li>Göteborg</li><li>Helsinki</li><li>Leyde</li></settlement><orgName><li>Université d'Helsinki</li><li>Université de Göteborg</li></orgName></list><tree><country name="Finlande"><noRegion><name sortKey="Heiskanen, Annamari" sort="Heiskanen, Annamari" uniqKey="Heiskanen A" first="Annamari" last="Heiskanen">Annamari Heiskanen</name></noRegion><name sortKey="Aitio, Olli" sort="Aitio, Olli" uniqKey="Aitio O" first="Olli" last="Aitio">Olli Aitio</name><name sortKey="Hirvonen, Tia" sort="Hirvonen, Tia" uniqKey="Hirvonen T" first="Tia" last="Hirvonen">Tia Hirvonen</name><name sortKey="Impola, Ulla" sort="Impola, Ulla" uniqKey="Impola U" first="Ulla" last="Impola">Ulla Impola</name><name sortKey="Laine, Jarmo" sort="Laine, Jarmo" uniqKey="Laine J" first="Jarmo" last="Laine">Jarmo Laine</name><name sortKey="Laitinen, Anita" sort="Laitinen, Anita" uniqKey="Laitinen A" first="Anita" last="Laitinen">Anita Laitinen</name><name sortKey="Lehenkari, Petri" sort="Lehenkari, Petri" uniqKey="Lehenkari P" first="Petri" last="Lehenkari">Petri Lehenkari</name><name sortKey="Natunen, Jari" sort="Natunen, Jari" uniqKey="Natunen J" first="Jari" last="Natunen">Jari Natunen</name><name sortKey="Natunen, Suvi" sort="Natunen, Suvi" uniqKey="Natunen S" first="Suvi" last="Natunen">Suvi Natunen</name><name sortKey="Olonen, Anne" sort="Olonen, Anne" uniqKey="Olonen A" first="Anne" last="Olonen">Anne Olonen</name><name sortKey="Saarinen, Juhani" sort="Saarinen, Juhani" uniqKey="Saarinen J" first="Juhani" last="Saarinen">Juhani Saarinen</name><name sortKey="Salo, Hanna" sort="Salo, Hanna" uniqKey="Salo H" first="Hanna" last="Salo">Hanna Salo</name><name sortKey="Satomaa, Tero" sort="Satomaa, Tero" uniqKey="Satomaa T" first="Tero" last="Satomaa">Tero Satomaa</name><name sortKey="Tiitinen, Sari" sort="Tiitinen, Sari" uniqKey="Tiitinen S" first="Sari" last="Tiitinen">Sari Tiitinen</name><name sortKey="Valmu, Leena" sort="Valmu, Leena" uniqKey="Valmu L" first="Leena" last="Valmu">Leena Valmu</name><name sortKey="Valmu, Leena" sort="Valmu, Leena" uniqKey="Valmu L" first="Leena" last="Valmu">Leena Valmu</name></country><country name="Suède"><region name="Götaland"><name sortKey="Miller Podraza, Halina" sort="Miller Podraza, Halina" uniqKey="Miller Podraza H" first="Halina" last="Miller-Podraza">Halina Miller-Podraza</name></region></country><country name="Pays-Bas"><region name="Hollande-Méridionale"><name sortKey="Wuhrer, Manfred" sort="Wuhrer, Manfred" uniqKey="Wuhrer M" first="Manfred" last="Wuhrer">Manfred Wuhrer</name></region><name sortKey="Deelder, Andre M" sort="Deelder, Andre M" uniqKey="Deelder A" first="André M." last="Deelder">André M. 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